pH Sensor Patches, Probes and CuvettesInstallation and Operation ManualDocument Number pHSENSOR-TRANS-REFLECT-02-201111Offices: Ocean Optics, Inc. Wor
1: Products4 PHSENSOR-TRANS-REFLECT-201111Performance SpecificationsSpecification Ocean Optics pH Probe ValuepH range 5 – 9Analytical Wavelength 620nm
1: ProductsPHSENSOR-TRANS-REFLECT-201111 5Ocean Optics VIS/NIR desktop or Jaz spectrometer (slit size 200μm)Light source (VIS/NIR: HL-2000, LS-1, LLS
1: Products6 PHSENSOR-TRANS-REFLECT-201111► Procedure1. Peel the liner backing from the reflective pH sensor patch, and firmly adhere the patch to the
1: ProductsPHSENSOR-TRANS-REFLECT-201111 72. Continue streaming jets of liquid onto the patch until the entire backside looks uniformly dark and wet.
1: Products8 PHSENSOR-TRANS-REFLECT-201111Smart pH CuvettesSensor System ComponentsCUV-UV Cuvette Holder or Jaz Direct-attach Cuvette HolderOptical fi
1: ProductsPHSENSOR-TRANS-REFLECT-201111 9For field measurements, an SD card with the pH software is required for the Ocean Optics handheld Jaz spectr
1: Products10 PHSENSOR-TRANS-REFLECT-201111Storage/LifetimeCuvettes can be stored dry at room temperature for any amount of time. As a rule, once the
PHSENSOR-TRANS-REFLECT-201111 11Chapter 2Desktop Software Installation and OperationCalibrating the pH Sensor SystemThe pH sensor patches and cuvettes
2: Desktop Software Installation and Operation12 PHSENSOR-TRANS-REFLECT-2011115. Enter your Experimental parameters: Acquisition Wavelength, Baseline
2: Desktop Software Installation and OperationPHSENSOR-TRANS-REFLECT-201111 13Smart pH Cuvettes:Use a dropper or pipette to add buffer to the cuvette.
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2: Desktop Software Installation and Operation14 PHSENSOR-TRANS-REFLECT-20111112. You are now ready to take pH measurements. See Taking pH Measurement
2: Desktop Software Installation and OperationPHSENSOR-TRANS-REFLECT-201111 156. Take a low pH reference spectrum at pH 1.0. To do this for your speci
2: Desktop Software Installation and Operation16 PHSENSOR-TRANS-REFLECT-201111Smart pH Cuvettes:Remove the pH 1 buffer with a pipette, without moving
2: Desktop Software Installation and OperationPHSENSOR-TRANS-REFLECT-201111 173. Click one or all of the following buttons: Reset: Clears the table a
2: Desktop Software Installation and Operation18 PHSENSOR-TRANS-REFLECT-2011112. Get the integration time. To do this, expose the sensor to pH 1 buffe
2: Desktop Software Installation and OperationPHSENSOR-TRANS-REFLECT-201111 198. Take a low pH reference spectrum at pH 1.0. This was exposed to the s
2: Desktop Software Installation and Operation20 PHSENSOR-TRANS-REFLECT-201111Performing a User Calibration with Jaz► Procedure1. Select the Setup/Cal
2: Desktop Software Installation and OperationPHSENSOR-TRANS-REFLECT-201111 215. Enter the baseline wavelength using the Jaz keys. Then click Accept.6
2: Desktop Software Installation and Operation22 PHSENSOR-TRANS-REFLECT-20111110. Take a high reference spectrum for pH 11.0. To do this, expose the s
2: Desktop Software Installation and OperationPHSENSOR-TRANS-REFLECT-201111 23Take a Single pH Measurement and Display It to the Jaz Screen► Procedure
PHSENSOR-TRANS-REFLECT-201111 iTable of ContentsAbout This Manual...
2: Desktop Software Installation and Operation24 PHSENSOR-TRANS-REFLECT-201111Take Continuous pH Measurements and Write Them to a Data File► Procedure
PHSENSOR-TRANS-REFLECT-201111 25Appendix AAlgorithms UsedpH CalculationSamplepHSampleAbsAbsAbsSlopepKpH11log*…where AbsSample is the sample
A: Algorithms Used26 PHSENSOR-TRANS-REFLECT-201111…for each of the buffers. The y-axis shows the pH value of the buffers. This generates a plot such a
PHSENSOR-TRANS-REFLECT-201111 27Appendix BTroubleshootingOverviewThe following are common sources of error. Transmissive pH ProbesProblem Possible Cau
B: Troubleshooting28 PHSENSOR-TRANS-REFLECT-201111Problem Possible Cause/Suggested SolutionUnsteady and improper setupIf the vessel or probe has play
PHSENSOR-TRANS-REFLECT-201111 29IndexAalgorithms, 25pH calculation, 25resetting pK and slope, 25temperature compensation, 25Ccalibration, 11using Spec
Index30 PHSENSOR-TRANS-REFLECT-201111SSD cardwrite continous pH measurements, 24setupreflective pH patches/probe, 5Smart Cuvettes, 9transmissive pH pa
Table of Contentsii PHSENSOR-TRANS-REFLECT-201111Appendix B: Troubleshooting...27Overview ...
PHSENSOR-TRANS-REFLECT-201111 iiiAbout This ManualDocument Purpose and Intended AudienceThis document provides you with installation and operation ins
About This Manualiv PHSENSOR-TRANS-REFLECT-201111You can access documentation for Ocean Optics products by visiting our website at http://www.oceanopt
PHSENSOR-TRANS-REFLECT-201111 1Chapter 1ProductsOverviewOcean Optics’ fully integrated pH systems provide full spectral analysis to help eliminate err
1: Products2 PHSENSOR-TRANS-REFLECT-201111Ocean Optics VIS/NIR desktop or Jaz spectrometer (slit size 25μm)Light source (VIS/NIR: HL-2000, LS-1, LLS S
1: ProductsPHSENSOR-TRANS-REFLECT-201111 35. The cap for the transmissive probe screws off. The probe uses a thin sensing film with adhesive on one si
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