Ocean-optics S2000 Manuel d'utilisateur

Operating Manualand User’s GuideS2000 Miniature Fiber Optic Spectrometers and AccessoriesOffices: East Coast380 Main Street, Dunedin, Fla., USA727.733

Configure Data Acquisition Dialog Box Finally, select Spectrum | Configure Data Acquisition from the menu to open the Configure Data Acquisitiondialog

Base Address Settings for the ADC1000 and PC2000 To change the Base Address settings on the ADC1000 and PC2000, see the bank of switches on the A/D bo

Interrupt Request Settings for the ADC1000 and PC2000 To change the IRQ settings on the ADC1000 and PC2000, see the bank of switches on the A/D board:

Appendix B: Adjusting the Focus for CollimatingLenses In order to obtain accurate data, the light entering and exiting a sample by means of a fiber/co

Appendix C: Calibrating the Wavelength of theSpectrometer The following describes how to calibrate the wavelength of your spectrometer. Though each sp

4. Now you are ready to calculate the wavelength calibration coefficients.In your spreadsheet program, find thefunctions to perform linear regressions

Appendix D: S2000 Pin-outs and JumpersThe average user would not normally need to know about the interconnect scheme of the S2000, as the cablessuppli

Appendix D- 106 -J2 Pin DB-15 (Female) Description1 Single StrobeTTL output signal used to pulse a strobe that is high at the start of eachintegration

Continuous Strobe RateThe continuous strobe rate is controlled by the A/D converter frequency input to the spectrometer and the positionof a jumper in

Appendix E: PC2000 Pin-outs and JumpersThe average user would not normally need to know about the interconnect scheme of the S2000, as thecables suppl

Appendix F: External Triggering Our S2000 and S1024DW Spectrometers, when used withOOIBase32 Spectrometer Operating Software, provide fourmethods of a

S2000 Miniature Fiber Optic SpectrometersOur second-generation miniature fiber opticspectrometer, the S2000, couples a low-cost,high-performance 2048-

source to supply the voltage, as it is based on a referenced ground and your reference may be different fromours. Using Pin 3 to supply voltage ensure

Configure Data Acquisition from the menu.4. Select Spectrum | Configure Data Acquisition from the menu. Choose the External Trigger page and selectSyn

4. Once you have selected the integration time, note the configuration of the pins in theJumper Block 4 column of the chart.5. Remove your spectromete

S2000 Specifications S2000 Miniature Fiber Optic Spectrometers-12-Absolute Maximum RatingsVCC+ 5.5 VDCVoltage on any pin VCC + 0.2 VDCPhysical Specifi

S2000 Board Layout H1 Header Block connecting analog signals from slave spectrometer channel(s) to the Master spectrometer channelH2 Header Block cont

A/D ConvertersThis section covers the basic installation instructions for our A/Dconverters: ADC500, ADC1000 (and PC2000), SAD500, and DAQ700.Because

ADC500 ISA-bus A/D ConverterThe ADC500 ISA-BUS A/D CONVERTER is a 12-bit, 8-channel ISA-bus analog-to-digital converter card thatconnects our spectrom

Windows NT Users:Find Available Base Address and IRQ Settings1. Go to Start | Programs | Administrative Tools (Common) | Windows NT Diagnostics.2. In

Run OOIBase32After you restart your computer, navigate to the OOIBase32 icon and select it. The first time you run OOIBase32after installation, you mu

Spectrometer Configuration Dialog BoxNow that OOIBase32 is running, you need to configure your system. Select Spectrometer | Configure from themenu. G

ADC1000 ISA-bus A/D Converter (and PC2000)The ADC1000 ISA-BUS A/D CONVERTER is a 12-bit, 8-channel, single-ended A/D card that connects ourspectromete

Copyright © 2000 Ocean Optics, Inc.All rights reserved. No part of this publication may be reproduced or transmittedin any form or by any means, elect

Windows NT Users:Find Available Base Address and IRQ Settings1. Go to Start | Programs | Administrative Tools (Common) | Windows NT Diagnostics.2. In

Run OOIBase32After you restart your computer, navigate to the OOIBase32 icon and select it. The first time you run OOIBase32after installation, you mu

Spectrometer Configuration Dialog BoxNow that OOIBase32 is running, you need to configure your system. Select Spectrometer | Configure from themenu. G

SAD500 Serial Port InterfaceThe SAD500 SERIAL PORT INTERFACE is a microprocessor-controlled A/D converter for serial port connection orstand-alone ope

Configure Hardware Dialog BoxNext, the Configure Hardware dialog box opens. Theparameters in this dialog box are usually set only once -- whenOOIBase3

Save the spectrometer configuration file by choosing Spectrometer | Save Configuration As from the OOIBase32menu. You can rename the file or use the d

DAQ-700 PCMCIA A/D ConverterThe DAQ-700 PCMCIA A/D CONVERTER is a 12-bit analog-to-digital converter card that connects our spectrometersto notebook P

For Windows 95/98 Users:Configure the DAQ-7001. If you hear the “happy” sound, click Start, and select Settings | Control Panel.2. Double-click the Sy

8. While making this change, notice the Conflict information area at the bottom. Make sure you choose a value thatsays No devices are conflicting. If

Run OOIBase32After you restart your computer, navigate to the OOIBase32 icon and select it. The very first time you runOOIBase32 after installation, y

Table of ContentsIntroduction ...

Spectrometer Configuration Dialog BoxNow that OOIBase32 is running, you need to configure your system. Select Spectrometer | Configure from themenu. G

OOIBase32 Spectrometer Operating Software OOIBASE32 SPECTROMETER OPERATING SOFTWARE is our next generation of operating software for all OceanOptics s

Other advanced features provide you with several data collection options. You have the ability to independentlystore and retrieve dark, reference, sam

Light SourcesOcean Optics light sources provide illumination and excitation in the UV, VIS or Shortwave NIR range forabsorbance, reflection and fluore

MINI-D2T Miniature Deuterium Tungsten Light SourceThe MINI-D2T MINIATURE DEUTERIUM TUNGSTEN LIGHT SOURCE combines the continuous spectrum of an RF-cou

4. When you want to control the MINI-D2T through OOIBase32 Spectrometer Operating Software, select ordeselect the Strobe Enable box in the Acquisition

D-1000 Deuterium Light Source The D-1000 DEUTERIUM LIGHT SOURCE is a high-output, high stability fiber optic light source optimized for theUV. The D-1

8. Lift up the black protective shutter and disconnect your optical fiber from the SMA connector.9. Replace the red plastic cap over the SMA connector

DT-1000 Deuterium Tungsten Halogen Light SourceThe DT-1000 DEUTERIUM TUNGSTEN HALOGEN LIGHT SOURCE combines the continuous spectrum of adeuterium UV l

Operating the Deuterium Source1. To power the deuterium lamp, push in the UV Start button located on the front panel. This white button turnson the de

Fiber Optic Probes and Accessories...83R200 Reflection Probes...

4. Gently grip the top of the bulb and pull it away from the cylindrical unit. Discard the bulb.5. Insert the new bulb into the top of the cylinder, b

LS-1 Tungsten Halogen Light Source The LS-1 TUNGSTEN HALOGEN LIGHT SOURCE is a versatile, white-light lamp utilized for the VIS-Shortwave NIR(360 nm-2

4. Locate the two set screws at the back of the lamp, one above each back leg. These two screws keep the twohalves of the lamp together. Remove the tw

PX-2 Pulsed Xenon Lamp The PX-2 PULSED XENON LAMP is a high flash rate, short-arc xenon lamp for applications involving absorbance,reflection, fluores

operating software. To achieve a constant number of flashes per integration cycle, the integration time must be amultiple of those shown in the follow

LS-450 Blue LED Pulsed Light Source The LS-450 BLUE LED PULSED LIGHT SOURCE is a compact, low-cost light-emitting diode that produces pulsed orcontinu

R-LS-450 Rack-mount Blue LED Pulsed Light Source The R-LS-450 BLUE LED PULSED LIGHT SOURCE is a compact, low-cost light-emitting diode that produces p

Jumper Block 3 (JP3)There are two sets of pins in JP3. The jumper position here determines the source of control for the R-LS-450:manual or remote con

You can control the pulses per second of the R-LS-450 through the Flash Delay function in the OOIBase32software if:" The switch is turned to puls

HG-1 Mercury Argon Calibration Source The HG-1 MERCURY ARGON CALIBRATION SOURCE is a wavelength calibration source for UV-VIS-ShortwaveNIR spectrophot

IntroductionOcean Optics miniature fiber optic spectrometers and accessories have revolutionized the analyticalinstrumentation market by dramatically

1. After placing OOIBase32 into Scope Mode, take a spectrum of your light source. Adjust the integration timeuntil there are several peaks on the scre

6. The numbers of importance are indicated in the above figure. You will need to record the Intercept as well asthe First, Second, and Third Coefficie

LS-1-CAL Calibrated Light Source The LS-1-CAL CALIBRATED LIGHT SOURCE for the VIS-Shortwave NIR (300-1050 nm) is a tungsten halogenlight source that p

6. Find the on/off switch on the back of the lamp and turn the lamp on.7. Let the lamp warm up for at least 15 minutes before using.8. Insert the disk

Sampling Chambers Ocean Optics offers a comprehensive line of compact, low-cost sampling chambers for a variety of UV-VIS-Shortwave NIR applications.

CUV-UV, CUV-VIS Cuvette Holders The CUV-UV and CUV-VIS CUVETTE HOLDERS for 1-cm path length cuvettes couple via SMA-terminated opticalfibers to our sp

Specifications* Though the VIS-NIR lens is optimized for use to 2 µm, it can be configured to "see" only to 1100 nm with ourS2000 spectromet

CUV-UV-10, CUV-VIS-10 Cuvette Holders The CUV-UV-10 and CUV-VIS-10 CUVETTE HOLDERS for 10-cm cuvettes couple to our spectrometers and lightsources to

CUV-ALL 4-way Cuvette Holder The CUV-ALL 4-WAY CUVETTE HOLDER for 1-cm path length cuvettes has four collimating lenses that couple tooptical fibers,

Installing Filters1. Loosen the filter clamping screw with the provided screwdriver.2. Insert the filter into the filter slot. The filter slot can acc

OOIBase32 Spectrometer Operating Software is our next generation of operating software for all Ocean Opticsspectrometers. OOIBase32 is a 32-bit, user-

CUV-FL-DA Direct Attach Cuvette Holder The CUV-FL-DA DIRECT ATTACH CUVETTE HOLDER attaches directly to our light sources and couples viaSMA-terminated

SpecificationsSampling Chambers: CUV-FL-DA-61-Path length: 1 cmDimensions: 1.4” x 1.4” x 1.0” (L x W x H)Material: Black anodized aluminumHole diamete

ISS Integrated Sampling SystemThe ISS INTEGRATED SAMPLING SYSTEM is a fully integrated 1-cm cuvette holder and tungsten halogen light source.It couple

8. Tighten the set screw on the bottom of the lamp.9. Close the two halves without pinching the wires. Replace the two screws at the back of the lamp.

ISS-2 Integrated Sampling SystemThe ISS-2 INTEGRATED SAMPLING SYSTEM is a fully integrated 1-cm cuvette holder and tungsten halogen lightsource. It co

6. Gently separate the two halves of the lamp and pull the bulb out of its housing. 7. Detach the wire and socket from the lamp leads. Remove bulb uni

ISS-UV-VIS Integrated Sampling SystemThe ISS-UV-VIS INTEGRATED SAMPLING SYSTEM is a combination of a RF deuterium source with a tungstenbulb connected

Operating the ISS-UV-VIS through Software1. Connect the 15-pin accessory cable from the spectrometer to the ISS-UV-VIS.2. Find the switch on the back

FHS-UV and FHS-VIS In-line Filter HoldersThe FHS-UV and FHS-VIS IN-LINE FILTER HOLDERS are low-cost spectrophotometric accessories for fast,convenient

SpecificationsSampling Chambers: FHS-UV, FHS-VIS-69-FHS-VIS Collimating lenses (VIS-NIR):BK 7 glass (~360 nm - 2 µm*)5 mm diameterf/2FHS-UV Collimatin

Packing List A packing list comes with your order. It is located inside a plastic bag attached to the outside of the shipment box.The invoice is maile

LPC Long Pass Flow CellsLPC LONG PASS FLOW CELLS from Ocean Optics couple to the company's high-sensitivity fiber opticspectrometers and compact

Assessing if the LPC is Free of ParticlesFluids need to be relatively particle-free. Particles larger than 20 µm can be trapped inside the tubing and

CUV-CCE Electrophoresis Sample CellThe CUV-CCE ELECTROPHORESIS SAMPLE CELL for chromatography and capillary electrophoresis is anoptical fixture for m

Checking the Alignment1. With the spectrometer running, observe the signal inScope Mode. When the optical window is properlypositioned, you can see a

Sampling Optics Ocean Optics offers numerous spectroscopic accessories. A short description for each accessory featured in thismanual is listed below.

74-UV, 74-VIS Collimating Lenses In order to obtain accurate data, the light entering the sample and the light collected after exiting the sample must

74-90-UV Right Angle Reflector The 74-90-UV RIGHT ANGLE REFLECTOR is a 3/8-24 threaded black anodized aluminum assembly for mountingcollimating lenses

74-OPM Optical Post Mount The 74-OPM OPTICAL POST MOUNT is a 3/8-24 threaded black anodized aluminum assembly used for mountingcollimating lenses on b

74-ACH Adjustable Collimating Lens Holder The 74-ACH ADJUSTABLE COLLIMATING LENS HOLDER is a versatile assembly for mounting lenses at multiplepositio

FVA-UV Fiber Optic Variable Attenuator The FVA-UV FIBER OPTIC VARIABLE ATTENUATOR is an opto-mechanical device that controls the amount of lightenteri

Quick StartThe S2000 is easy to set up, allowing the user to start collecting data within minutes. The three pages in thissection provide brief instru

WS-1 Diffuse Reflectance Standard The WS-1 DIFFUSE REFLECTANCE STANDARD is a compact physical standard for use in performing diffusereflectance measur

ISP-REF Integrating Sphere The ISP-REF INTEGRATING SPHERE is an illuminated sampling optic that couples via optical fiber to Ocean Opticsminiature fib

FOIS-1 Fiber Optic Integrating Sphere The FOIS-1 FIBER OPTIC INTEGRATING SPHERE is a sampling optic that accepts light energy through its 0.375"i

Fiber Optic Probes and Accessories Ocean Optics fiber optic probes couple to our miniature fiber optic spectrometers and light sources to create a var

R200 Reflection Probes R200 REFLECTION PROBES couple to Ocean Optics miniature fiber optic spectrometers and light sources to createsmall-footprint op

RPH-1 Reflection Probe HolderThe RPH-1 REFLECTION PROBE HOLDER is an anodized aluminum platform with machined holes at 45° and 90°to hold our R200 Ref

T300-RT-UV-VIS Transmission Dip ProbeThe T300-RT-UV/VIS TRANSMISSION DIP PROBE couples to our spectrometers and light sources to create small-footprin

CC-3 Cosine-corrected Irradiance Probes The CC-3 COSINE-CORRECTED IRRADIANCE PROBES are spectroradiometric sampling optics designed to collectradiatio

FL-400 Flame-resistant Fiber ProbeThe FL-400 FLAME-RESISTANT FIBER PROBE is a heat-resistant fiber optic probe that couples to Ocean Opticsminiature f

Optical Fiber Assemblies Ocean Optics offers an extensive line of optical fibers and accessories -- including patch cords, bifurcatedassemblies, bushi

4. Note these values. When you first run OOIBase32, you must enter these values in the “Configure Hardware”dialog box.If your A/D converter is the SAD

Splice Bushings, Bulkhead Fittings Splice bushings are used to couple two SMA-terminated fibers together. Simply screw each fiber into the splicebushi

Experiment Tutorial Before you begin your experiment, double-check that you have correctly installed your A/D converter, installedthe operating softwa

Absorbance Experiments Absorbance spectra are a measure of how much light is absorbed by a sample. For most samples, absorbance islinearly related to

Transmission Experiments Transmission is the percentage of energy passing through a system relative to the amount that passes through thereference. Tr

Reflection Experiments Reflection is the return of radiation by a surface, without a change in wavelength. The reflection may be:" Specular, in w

Relative Irradiance Experiments Irradiance is the amount of energy at each wavelength from a radiant sample. In relative terms, it is the fractionof e

Time Acquisition Experiments Our OOIBase32 Spectrometer Operating Software allows you to perform time acquisition experiments. Timeacquisition experim

7. Access the Time AcquisitionConfiguration dialog box by selectingTime Acquisition | Configure |Configure Acquisition from the menu." Enable Str

Appendix A: Changing the A/D Converter SettingsBase Address Settings for the ADC500 To change the Base Address settings on the ADC500 board, see the b

Interrupt Request Settings for the ADC500 To change the IRQ settings on the ADC500 board, see the bank of 4 dip switches labeled SW2. In the defaultse